Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?
![View of The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform | Journal of Biological Methods View of The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform | Journal of Biological Methods](https://www.jbmethods.org/public/journals/1/cover_article_289_en_US.jpg)
View of The answer depends on the question: Optimal conditions for western blot characterization of muscle collagen type 1 depends on desired isoform | Journal of Biological Methods
![China Western Blotting SDS-Page Transfer Buffer Powder - China SDS-Page Buffer, SDS-Page Transfer Buffer China Western Blotting SDS-Page Transfer Buffer Powder - China SDS-Page Buffer, SDS-Page Transfer Buffer](https://image.made-in-china.com/2f0j00qZulYTUFOrkP/Western-Blotting-SDS-Page-Transfer-Buffer-Powder.jpg)
China Western Blotting SDS-Page Transfer Buffer Powder - China SDS-Page Buffer, SDS-Page Transfer Buffer
![Optimization of western blotting for the detection of proteins of different molecular weight | BioTechniques Optimization of western blotting for the detection of proteins of different molecular weight | BioTechniques](https://www.future-science.com/cms/10.2144/btn-2019-0124/asset/images/medium/figure2.gif)